Titlebook: Advanced Methods in Structural Biology; Toshiya Senda,Katsumi Maenaka Book 2016 Springer Japan 2016 Protein science.Experimental protocols

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2.2.1.6 Arrangement of figures and tables,this chapter, we explain the basic concepts of these expression systems and provide examples of advanced new techniques including baculovirus-silkworm expression and HEK293 GnTI-cell expression to confer uniformed .-glycans suitable for structural studies.

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2.2.1.6 Arrangement of figures and tables,ion bodies are described. Here, the mechanism of protein refolding, the isolation of inclusion bodies, the choice of solubilizing materials, the refolding step, and the effects of additives while refolding are included. The selection of various strategies in these steps is discussed.