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Titlebook: Adipose-Derived Stem Cells; Methods and Protocol Bruce A. Bunnell,Jeffrey M. Gimble Book 2018Latest edition Springer Science+Business Media

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发表于 2025-3-21 19:52:57 | 显示全部楼层 |阅读模式
期刊全称Adipose-Derived Stem Cells
期刊简称Methods and Protocol
影响因子2023Bruce A. Bunnell,Jeffrey M. Gimble
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Adipose-Derived Stem Cells; Methods and Protocol Bruce A. Bunnell,Jeffrey M. Gimble Book 2018Latest edition Springer Science+Business Media
影响因子This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Adipose-Derived Stem Cells: Methods and Protocols, Second Edition. is a valuable resource for anyone interested in learning more aboutthe scientific advances in the field of ASC biology and application..
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书目名称Adipose-Derived Stem Cells读者反馈学科排名




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Gewinnung und Reindarstellung von Proteinenechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells along with protocols for inducing adipogenesis in this cell population or isolating the adipose stromal vascular fraction cells for flow cytometric analysis.
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Antigene, Antikörper und KomplementASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.
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Mareike Hennings,Rosemarie Mielkeheir ability to undergo facile differentiation into adipose tissue, their angiogenic properties, and their ability to express and secrete multiple growth factors. This chapter discusses the isolation and characterization of human ASCs, its expansion in vitro, and relevant in vivo models for adipose tissue engineering.
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Mareike Hennings,Rosemarie Mielkets (CPAs), freezing equipment, and obviously, storage in liquid nitrogen. The purpose of this chapter is to present a general overview of cryopreservation storage techniques and the optimal protocols/results obtained in our laboratory for long-term storage of adult stem cells using freezing storage.
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Bausteine der kindlichen Entwicklungding that growing cells on plastic surfaces cannot truly recapitulate 3D in vivo conditions. Here we describe a novel method for generating and culturing rabbit ASCs as scaffold-free 3D cell aggregates using micropatterned wells via a forced aggregation technique.
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Nachweis und Bestimmung einzelner Elementeical clearing technique which facilitates the detailed characterization of whole-mount samples from skin and adipose tissues (ex vivo tissues and in vitro tissue-engineered substitutes) when combined with spectral confocal microscopy and quantitative analysis on image renderings.
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