Titlebook: Adenovirus Methods and Protocols; Volume 2: Ad Protein William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 PCR.RN

钢盔

CLAMP

Methods in Molecular Medicinehttp://image.papertrans.cn/a/image/144887.jpg

不能妥协

Franco H. Falcone,Bernhard F. GibbsHere we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts from virus-infected cells and how to analyze RNA splicing in vitro using T7 RNA polymerase-derived splicing substrate RNAs.

无聊的人

Franco H. Falcone,Bernhard F. Gibbsromoter(s) of interest. The latter parameter is assessed by quantification of viral DNA by hybridization of membrane-bound DNA following purification of DNA from nuclear fractions of adenovirus-infected cells. Two alternative protocols, primer extension and quantitative reverse transcription polymer

鲁莽

AUGUR

Edward F. Knol,Bernhard F. Gibbsem. Here we present a collection of in vitro methods to study RNAi and the effect of an adenovirus infection on RNAi. We describe methods to measure the two key enzymatic complexes involved in RNAi: Dicer and RISC.

独轮车

GORGE

发微光

肿块

Rick A. Adams,Scott C. Pedersenon between a monoclonal antibody and a protein of interest. Providing that the antibody—protein interaction does not interfere with the ability of the protein to interact with other proteins in a cell lysate, a protein complex containing the antibody, the protein of interest, and its interacting pro