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Titlebook: Adenovirus Methods and Protocols; Volume 1: Adenovirus William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 DNA.EL

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发表于 2025-3-21 19:35:56 | 显示全部楼层 |阅读模式
期刊全称Adenovirus Methods and Protocols
期刊简称Volume 1: Adenovirus
影响因子2023William S. M. Wold,Ann E. Tollefson
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发行地址Detailed description of quality control testing of vectors.Methods for determination of virus titers.Sensitive assays for testing viral DNA presence in samples.Developing new, permissive immunocompete
学科分类Methods in Molecular Medicine
图书封面Titlebook: Adenovirus Methods and Protocols; Volume 1: Adenovirus William S. M. Wold,Ann E. Tollefson Book 2007Latest edition Humana Press 2007 DNA.EL
影响因子.Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise...Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-a
Pindex Book 2007Latest edition
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Kristina Reiss,Gerald Schmiederuction of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.
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,Einfüuhrung in die Systemtheorie,hods for generating subcutaneous tumors in cotton rats and assessing the efficacy of Ad vectors upon intratumoral injection. Also, we discuss procedures for determining the biodistribution of a replicating Ad in cotton rats.
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Fazit zu den wichtigsten Steuerarten,luding human cells, poorly. However, genetic modification of capsid proteins (e.g., fiber, pIX) has helped in increasing the utility of BAdV-3 as a vector for transducing nonbovine cells. Here, we will describe the methods used to construct recombinant BAdV-3 expressing chimeric fiber or chimeric pIX proteins.
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Book 2007Latest edition, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad ve
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https://doi.org/10.1007/978-3-658-23827-8ffords one the opportunity to “detarget” adenovirus, to retarget adenovirus, and to alter immune recognition. In this chapter, we are presenting a terminal exon-replacement strategy that can be used to genetically manipulate capsid proteins expressed from the MLTU. An emphasis will be placed on manipulations of fiber as an intact terminal exon.
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