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Titlebook: Adeno-Associated Virus Vectors; Design and Delivery Michael J. Castle Book 2019 Springer Science+Business Media, LLC, part of Springer Natu

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发表于 2025-3-21 17:14:07 | 显示全部楼层 |阅读模式
期刊全称Adeno-Associated Virus Vectors
期刊简称Design and Delivery
影响因子2023Michael J. Castle
视频videohttp://file.papertrans.cn/145/144869/144869.mp4
发行地址Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Adeno-Associated Virus Vectors; Design and Delivery Michael J. Castle Book 2019 Springer Science+Business Media, LLC, part of Springer Natu
影响因子This volume provides a complete and timely guide to the use of adeno-associated virus (AAV)  vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. .Authoritative and comprehensive, .Adeno-Associated Virus Vectors: Design and Delivery. aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of
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发表于 2025-3-21 22:51:41 | 显示全部楼层
Volker Arolt,Christian Reimer,Horst Dillings cellular miRNA backbones, choice between polymerase II and III promoters, and the potential impact of these factors on toxicity as it relates to off-targeting and to saturation of the endogenous RNAi machinery.
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https://doi.org/10.1007/978-3-540-49927-5cuss the prevalent routes of administration to deliver rAAV to the CNS via intravenous (IV) injection in mice. We will highlight key considerations for using rAAV, and the advantages and disadvantages of each administration method. We will also briefly discuss intravenous delivery in larger animal m
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Volker Arolt,Christian Reimer,Horst Dillingntified via rational design or directed evolution have offered only incremental improvements, and have failed to promote pan-inner retinal transduction or significant outer retinal transduction beyond the fovea. Problems with retinal transduction by Ivt-delivered AAV include dilution in the vitreous
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