Titlebook: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis; Thomas Meier,Falk Fahrenholz Book 1996 Birkhäuser Verlag 1996 DNA.PAC.PCR.S

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Anne J. Alexander,Moira van Staadenractions between cell surface molecules. However, such studies often require the use of chemical cross-linking agents to cross-link and preserve molecular associations upon cell disruption, and subsequent analysis of specific molecules in the cell lysate by immunoprecipitation and SDS-polyacrylamide

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Perspectives in Vertebrate Sciencebinding activity which were detectable with high sensitivity using enzyme-conjugated ExtrAvidin. Lectins biotinylated with biotin hydrazide via periodate-oxidized carbohydrate residues were less sensitive reagents in avidin-mediated assays; however, the latter method may serve for identification of

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Molecules for Microcontact Printing,otinylated with retention of their biological activities. Biotin-labeled SLPI was suitable as a standard in an ELISIA for the quantitation of human connective tissue serine proteinase inhibitors (SPIs) (2). Biotin-labeled PCTI-1 and aprotinin were sensitive probes for the detection of a range of ser

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Local Oxidation Nanolithography,e bloodstream. Such immunoerythrocytes have to circulate in the bloodstream for a prolonged time without elimination and possess high affinity to the target antigen. Streptavidin/biotin technology is useful for attachment of biotinylated antibodies (b-Ab) to biotinylated red blood cells (b-RBC). Mod

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Alternative Logics. Do Sciences Need Them? of RNAs or cDNAs and the functional effects of mutations. Usually, a natural and purified RNA or an mRNA transcript synthesized from cloned cDNA . with a phage RNA polymerase (SP6, T3, or 17) is added to an . translation system in order to program the synthesis of the encoded protein. . translation

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Alternative Logics. Do Sciences Need Them?tection of single-copy genes in Southern blots and low abundance mRNAs in Northern blots. Labelling of (c)DNA probes involves replacement of dTTP by either digoxigenin-dUTP or biotinylated dUTP. Here we describe experimental protocols for Northern blot analysis and for genomic Southern blot using a

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Outline of a Paraconsistent Category Theoryn of the Northwestern assay using biotinylated riboprobes. The method detects binding of biotinylated RNA to proteins fixed on nitrocellulose. Proteins are first separated by SDS-polyacrylamide gel electrophoresis (PAGE), transferred to the nitrocellulose membrane, and renatured. Therefore, generati