书目名称 | Zygotic Genome Activation |
副标题 | Methods and Protocol |
编辑 | Kiho Lee |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts.Includes supplementary material |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Thorough and cutting-edge, .Zygotic Genome Activation: Methods and Protocols .is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA.. |
出版日期 | Book 2017 |
关键词 | ZGA; Zygote; embryogenesis; epigenetics; genetic engineering; CRISPR |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-6988-3 |
isbn_softcover | 978-1-4939-8359-9 |
isbn_ebook | 978-1-4939-6988-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media LLC 2017 |