书目名称 | Yeast Surface Display | 编辑 | Michael W. Traxlmayr | 视频video | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Yeast Surface Display. serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experienc | 出版日期 | Book 2022 | 关键词 | Protein engineering; Yeast-displayed protein variants; Mammalian cells; Heterologous target protein anc | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-2285-8 | isbn_softcover | 978-1-0716-2287-2 | isbn_ebook | 978-1-0716-2285-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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