书目名称 | Xenopus | 副标题 | Methods and Protocol | 编辑 | Kris Vleminckx | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Xenopus: Methods and Protocols. is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping .Xenopus. at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.. | 出版日期 | Book 2018 | 关键词 | CRISPR; Cas9; X; tropicalis; TALEN; Frog models | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-8784-9 | isbn_softcover | 978-1-4939-9391-8 | isbn_ebook | 978-1-4939-8784-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2018 |
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