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Titlebook: 3D Bioprinting; Principles and Proto Jeremy M. Crook Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Bioprinte

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Eleonore Pauwels,Sarah W. Detonon of electrically stimulating cells in situ during and after tissue development. We and others have demonstrated the use of electrical stimulation (ES) to influence cell behavior and function for a more biomimetic approach to tissue engineering. Here, we detail a previously published method for 3D
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Giulio Maria Mancini,James Revillnstruct in tissue engineering. Different technologies have been reported for the fabrication of vasculature conduits, such as decellularized tissues and biomaterial-based blood vessels. Recently, bioprinting has also been considered as a promising method in vascular tissue engineering. In this work,
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Steven Andorka,Kira Rambow-HoescheleD multicellular spheroids, without requiring biomaterial. This is in contrast to traditional 3D bioprinting, which requires biomaterials to carry the cells to be bioprinted, such as a hydrogel or decellularized extracellular matrix. Here, we discuss principles of spheroid preparation for biomaterial
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Sunme Park,Kanggu Park,Euiseok Hwangcytes from skin biopsies. We generate 100 cm. of printed skin in less than 35 min. We analyze its structure using histological and immunohistochemical methods, both in in vitro 3D cultures and upon transplantation to immunodeficient mice. We have demonstrated that the printed skin is similar to norm
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Luís Sousa,Pedro M. Santos,Ana Aguiar Likewise, massive and ultimately catastrophic muscle cell loss occurs over time with progressive degenerative muscle diseases, such as the muscular dystrophies. Repair of volumetric loss of skeletal muscle requires replacement of large volumes of tissue to restore function. Repair of larger lesions
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Steven Andorka,Kira Rambow-Hoeschelepatially arrange them in 3D around magnet dots. Here, we report an M3DB methodology to generate salivary gland-like epithelial organoids from stem cells. These organoids possess a neuronal network that responds to saliva neurostimulants.
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