Titlebook: 2-D PAGE Map Analysis; Methods and Protocol Emilio Marengo,Elisa Robotti Book 2016 Springer Science+Business Media New York 2016 bioinforma

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Urbane öffentliche Räume als Aneignungsräumes resulting from the separation of protein mixtures. The method allows to obtain fundamental analytical information hidden in 2-D PAGE maps by spot overlapping, such as the number of proteins present in the sample and the mean standard deviation of the spots, describing the separation performance. I

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https://doi.org/10.1007/978-3-322-81029-8ts one of the bottlenecks in 2-DE studies. Over the years, different commercial and academic software packages have been developed for the analysis of 2-DE images. Each of these shows different advantageous characteristics in terms of quality of analysis. In this chapter, the characteristics of the

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Akteure einer zukunftsfähigen Entwicklungdetection or pixel-based methods. The Median Modified Wiener Filter (MMWF), a new nonlinear adaptive spatial filter, resulted to be a good denoising approach to use in practice with 2-DE. MMWF is suitable for global denoising, and contemporary for the removal of spikes and Gaussian noise, being its

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https://doi.org/10.1007/978-3-322-81029-8hing plays a fundamental role, in particular for the identification of proteins. We describe a simple and accurate method which allows to automatically and accurately match spots in 2-DE images. The method consists of simultaneously exploiting the distance between the spots, their intensity, and the

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https://doi.org/10.1007/978-3-322-81061-8ter spots among gels, are able to overcome the difficulties due to the low reproducibility of this analytical technique. Over the years, the research of new matching and warping mathematical methods has allowed the development of several routine applications of easy-to-use software. This chapter des

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