Malnutrition
发表于 2025-3-21 17:57:14
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有发明天才
发表于 2025-3-21 23:16:47
Use of a Golden Gate Plasmid Set Enabling Scarless MoClo-Compatible Transcription Unit Assembly,erate transcription units. Typically, type IIS enzymes are used, which generate four nucleotide overhangs. This results in small scar sequences in hierarchical DNA assemblies, which can affect the functionality of transcription units. However, there are enzymes that generate three nucleotide overhan
半球
发表于 2025-3-22 01:34:02
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inveigh
发表于 2025-3-22 07:54:54
Validation of Golden Gate Assemblies Using Highly Multiplexed Nanopore Amplicon Sequencing,libraries, resulting in assemblies of high combinatorial complexity where sequencing again becomes mandatory. Here, we present a detailed protocol for the use of a highly multiplexed dual barcode amplicon sequencing using the Nanopore sequencing platform for in-house sequence validation. The workflo
Ptosis
发表于 2025-3-22 09:47:56
Modular Golden Gate Assembly of Linear DNA Templates for Cell-Free Prototyping,tion of a repressor–promoter pair. By eliminating time-consuming transformation and cloning steps in cells and by taking advantage of modular cloning toolboxes, our cell-free prototyping workflow can produce data for large numbers of new assembled constructs within a single day.
convert
发表于 2025-3-22 14:29:05
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convert
发表于 2025-3-22 18:07:44
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BIDE
发表于 2025-3-22 23:33:47
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Lumbar-Spine
发表于 2025-3-23 03:37:40
Rosemarie Klemm,Dietrich D. Klemming the type of part that needs to be cloned, (2) designing primers for amplification, (3) performing polymerase chain reaction (PCR) amplification, (4) cloning of the fragments using Golden Gate cloning, and finally (5) sequencing of the part. For large standard parts, it is preferable to first clo
coagulation
发表于 2025-3-23 07:12:49
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