eustachian-tube
发表于 2025-3-23 12:49:02
Rollenkonzepte dynamischer Organisationen,, and modular cloning indexed plasmid library assembly. These upgrades enable a broad pool of users with varying levels of experience to readily implement advanced Golden Gate applications using low-cost, open-source lab robotics.
Tremor
发表于 2025-3-23 16:13:22
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思考
发表于 2025-3-23 21:05:10
Formulierung der Forschungsfragen,tion of a repressor–promoter pair. By eliminating time-consuming transformation and cloning steps in cells and by taking advantage of modular cloning toolboxes, our cell-free prototyping workflow can produce data for large numbers of new assembled constructs within a single day.
Arb853
发表于 2025-3-23 22:59:24
,Zur Struktur der Beschäftigten,nhance solubility and expression. The system allows visual candidate screening through mScarlet fluorescence and solubility tags are removable via TEV protease cleavage. We provide a comprehensive protocol encompassing oligonucleotide design, cloning, expression, His-tag affinity chromatography, and
复习
发表于 2025-3-24 05:27:42
https://doi.org/10.1007/978-3-7091-1232-8ly de-modify methylated or hydroxymethylated cytosines on phage DNA. The temporal removal of cytosine modification ultimately enables efficient DNA cleavage by Cas enzymes and facilitates mutagenesis. To streamline the application of the coupled TET-CRISPR-Cas system, we use Golden Gate cloning for
清唱剧
发表于 2025-3-24 09:47:19
https://doi.org/10.1007/978-90-481-3040-5ed plasmid backbone, for rapid and simple production of complex, multi-part assemblies. Insertion of a gene for superfolder green fluorescent protein, with selection by tetracycline resistance, into . strain MG1655 is used as an example but in principle the method can be tailored for virtually any m
声音刺耳
发表于 2025-3-24 11:19:30
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Irritate
发表于 2025-3-24 15:15:05
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radiograph
发表于 2025-3-24 22:16:48
Selection of Fusion-Site Overhang Sets for High-Fidelity and High-Complexity Golden Gate Assembly,ired order. Traditionally, fusion-site sequences are selected by using validated sets of overhang sequences or by applying a handful of semi-empirical rules to guide overhang choice. While these approaches allow dependable assembly of 6–8 fragments in one pot, recent work has demonstrated that compr
新陈代谢
发表于 2025-3-25 00:09:38
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