separate
发表于 2025-3-28 15:04:39
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figure
发表于 2025-3-28 21:03:43
,Was Sie aus diesem Buch lernen können,d construction of transcription units and multi-gene constructs. Importantly, its modular structure makes it compatible with laboratory automation, allowing for systematic and highly complex DNA assembly. Golden Gate cloning relies on type IIS enzymes that cleave an adjacent undefined sequence motif
载货清单
发表于 2025-3-29 02:59:58
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Outspoken
发表于 2025-3-29 06:01:14
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Malaise
发表于 2025-3-29 08:55:25
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META
发表于 2025-3-29 11:29:46
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拥护者
发表于 2025-3-29 18:02:20
Methodologische Vorbemerkungen,r delays by DNA synthesis vendors. Here, we describe a method for the assembly of small synthetic genes with repetitive elements: First, a gene of interest is split in silico into small synthons of up to 80 base pairs flanked by Golden Gate-compatible overhangs. Then synthons are made by oligo exten
假装是你
发表于 2025-3-29 22:51:31
,Zur Struktur der Beschäftigten,nd structural analyses of the purified samples. The demand for substantial biomolecule quantities often presents challenges, particularly for eukaryotic proteins. . expression systems have evolved to address these issues, offering advanced features such as solubility tags, posttranslational modifica
地名词典
发表于 2025-3-29 23:58:52
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渗透
发表于 2025-3-30 04:47:11
https://doi.org/10.1007/978-3-663-05454-2 cloning. While currently offering the most affordable source of synthetic DNA, oligo pools also come with limitations such as a maximum synthesis length (approximately 350 bases), a higher error rate compared to alternative synthesis methods, and the presence of truncated molecules in the pool due