archaeology
发表于 2025-3-23 13:28:06
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老人病学
发表于 2025-3-23 17:19:06
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nuclear-tests
发表于 2025-3-23 19:05:10
DNA Transfer (2) create DNA-containing complexes whose charge characteristics are compatible with DNA uptake by the target cell; or (3) result in the transient formation of pores in the plasma membrane of a cell exposed to an electric pulse, pores of sufficient size to allow DNA to enter the target cell.
热情赞扬
发表于 2025-3-23 22:48:44
Expression Cloningis type include selectable markers and oncogenes, as well as cytokines (Wong et al., 1985) and membrane proteins including cell-surface antigens (Aruffo and Seed, 1987) and cellular receptors (D’Andrea et al., 1989).
MUTED
发表于 2025-3-24 05:32:37
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蒸发
发表于 2025-3-24 08:48:47
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ANIM
发表于 2025-3-24 11:13:24
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苦笑
发表于 2025-3-24 16:05:04
Clinical Anatomy and Embryologylled incubator, an inverted microscope, a 4°C refrigerator, a −20°C freezer, and a liquid-nitrogen freezer for long-term cell storage. It is important that the flow hood, incubator, and microscope are located such that they are separate from the facilities used for the manipulation of bacterial or y
孵卵器
发表于 2025-3-24 22:55:08
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anachronistic
发表于 2025-3-25 03:11:58
https://doi.org/10.1007/978-981-10-0240-3tion can be infected with a recombinant retrovirus. All other means of stable transformation are less efficient. To facilitate the isolation of cells stably transformed with the DNA of interest, a gene encoding a dominant selectable marker is usually included in the transfection protocol. Such a gen