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发表于 2025-3-21 19:11:57
书目名称Gene Transfer and Expression影响因子(影响力)<br> http://figure.impactfactor.cn/if/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression影响因子(影响力)学科排名<br> http://figure.impactfactor.cn/ifr/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression网络公开度<br> http://figure.impactfactor.cn/at/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression网络公开度学科排名<br> http://figure.impactfactor.cn/atr/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression被引频次<br> http://figure.impactfactor.cn/tc/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression被引频次学科排名<br> http://figure.impactfactor.cn/tcr/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression年度引用<br> http://figure.impactfactor.cn/ii/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression年度引用学科排名<br> http://figure.impactfactor.cn/iir/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression读者反馈<br> http://figure.impactfactor.cn/5y/?ISSN=BK0381992<br><br> <br><br>书目名称Gene Transfer and Expression读者反馈学科排名<br> http://figure.impactfactor.cn/5yr/?ISSN=BK0381992<br><br> <br><br>
Irrepressible
发表于 2025-3-21 21:17:21
Vectorsrengths and weaknesses, many of which are presented in abbreviated form in Table 2-1. In my review of these creations, I will illustrate how they can be used, as well as provide sufficient information to allow you to incorporate your own gene or cDNA inserts into these vectors. Before I describe thi
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发表于 2025-3-22 01:22:41
Processing of Proteins Encoded by Transferred Genes study the gene product of interest. If that gene product is a protein molecule, you have a substantial armamentarium of methods at your disposal. In this section, I briefly describe (1) factors that can be experimentally manipulated to enhance mRNA translation, (2) the mechanism of protein processi
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发表于 2025-3-22 06:15:27
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发表于 2025-3-22 09:02:59
DNA Transfer (2) create DNA-containing complexes whose charge characteristics are compatible with DNA uptake by the target cell; or (3) result in the transient formation of pores in the plasma membrane of a cell exposed to an electric pulse, pores of sufficient size to allow DNA to enter the target cell.
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发表于 2025-3-22 13:08:01
Selection and Amplificationtion can be infected with a recombinant retrovirus. All other means of stable transformation are less efficient. To facilitate the isolation of cells stably transformed with the DNA of interest, a gene encoding a dominant selectable marker is usually included in the transfection protocol. Such a gen
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发表于 2025-3-22 19:05:42
Expression Cloningis type include selectable markers and oncogenes, as well as cytokines (Wong et al., 1985) and membrane proteins including cell-surface antigens (Aruffo and Seed, 1987) and cellular receptors (D’Andrea et al., 1989).
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发表于 2025-3-22 23:32:39
PCR-Based Expression chosen because it involves many of the types of problems one can run into when one chooses to pursue this approach. The solutions to these problems are also presented. In these experiments, we assembled a functional cDNA clone encoding a human leukocyte interferon a/d hybrid protein. We chose to sy
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发表于 2025-3-23 05:06:23
Assays for Gene Transferferred DNA by a Southern blot of genomic DNA or, if the transferred DNA has some unique sequence feature, by the polymerase chain reaction (PCR) carried out on genomic DNA. One may also monitor successful gene transfer by assaying for mRNA production by the exogenous DNA through Northern analysis of
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发表于 2025-3-23 08:51:39
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