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Autonomic Nervous System in the Neonateferred DNA by a Southern blot of genomic DNA or, if the transferred DNA has some unique sequence feature, by the polymerase chain reaction (PCR) carried out on genomic DNA. One may also monitor successful gene transfer by assaying for mRNA production by the exogenous DNA through Northern analysis of

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Book 1990A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.
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查看完整版本: Titlebook: Gene Transfer and Expression; A Laboratory Manual Michael Kriegler Book 1990 Stockton Press 1990 Calcium.DNA.nucleic acid.protein.RNA