legerdemain 发表于 2025-3-21 17:36:16

书目名称G Protein Signaling影响因子(影响力)<br>        http://figure.impactfactor.cn/if/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling影响因子(影响力)学科排名<br>        http://figure.impactfactor.cn/ifr/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling网络公开度<br>        http://figure.impactfactor.cn/at/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling网络公开度学科排名<br>        http://figure.impactfactor.cn/atr/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling被引频次<br>        http://figure.impactfactor.cn/tc/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling被引频次学科排名<br>        http://figure.impactfactor.cn/tcr/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling年度引用<br>        http://figure.impactfactor.cn/ii/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling年度引用学科排名<br>        http://figure.impactfactor.cn/iir/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling读者反馈<br>        http://figure.impactfactor.cn/5y/?ISSN=BK0380002<br><br>        <br><br>书目名称G Protein Signaling读者反馈学科排名<br>        http://figure.impactfactor.cn/5yr/?ISSN=BK0380002<br><br>        <br><br>

Ceremony 发表于 2025-3-22 00:06:13

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熟练 发表于 2025-3-22 01:34:56

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PALL 发表于 2025-3-22 07:54:57

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渐变 发表于 2025-3-22 11:19:23

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Contracture 发表于 2025-3-22 16:51:37

Book 2004ucture, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent

Contracture 发表于 2025-3-22 17:04:40

Expression and Purification of Soluble Adenylyl Cyclase from ,histidine tag (H6) is incorporated into the expression vector and utilized for affinity purification on a Ni-NTA column. Subsequently, an anion exchange column is employed to further purify the protein.

青石板 发表于 2025-3-22 21:59:48

Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cellsbecause AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are applicable to all nine isoforms of mammalian membrane-bound AC isoforms.

cortisol 发表于 2025-3-23 03:23:42

Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cellsibes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to examine the activation of G protein-coupled receptors (GPCRs), coupling of GPCRs to G proteins, and the interactions of G proteins to PLCβ.

Wernickes-area 发表于 2025-3-23 06:10:21

Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to Gα..
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查看完整版本: Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004