nitric-oxide
发表于 2025-3-26 21:04:58
G Protein Signaling978-1-59259-430-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
Exclaim
发表于 2025-3-27 01:24:46
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不感兴趣
发表于 2025-3-27 08:02:30
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hypnotic
发表于 2025-3-27 10:04:58
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Conduit
发表于 2025-3-27 17:32:29
Carol Anne Spreen,Chrissie Monaghanin for biochemical and biophysical analyses. Wild-type and mutant forms of Gα are easily produced for analysis of their intrinsic biochemical properties, as well as for reconstitution with receptors, effectors, regulators, and G protein βγ subunits. Methods are described for the expression of G.α an
Intersect
发表于 2025-3-27 21:04:25
Yukiko Yamaguchi,Nikhilesh Dholakiacombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.
beta-carotene
发表于 2025-3-27 22:57:15
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易于出错
发表于 2025-3-28 04:24:00
From Tre Styles to Trayvon Martinerol (DAG) and inositol 1,4,5-triphosphate (IP.). The PLCβ isoforms of PLCs are activated by G proteins after hormone or neurotransmitter stimulation of G protein-coupled receptors (GPCR). PLCe is a recently identified PLC isoform that is activated by Ras and Gβγ subunit although the physiological r
transdermal
发表于 2025-3-28 10:06:15
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Commodious
发表于 2025-3-28 10:50:12
https://doi.org/10.1007/978-3-662-09790-8r assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are