Lobotomy
发表于 2025-3-23 11:14:46
Selective Inhibition of G Protein-Mediated Pathways Using RGS Domainss through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that are mediated by the interacting G proteins. The method is particularly useful to differentiate between G.-mediated and G.-mediated pathways.
旧式步枪
发表于 2025-3-23 17:21:32
Carol Anne Spreen,Chrissie Monaghan)-tagged subunits using metal chelate chromatography. Modification of Gα with myristate can be recapitulated in . by expressing .-myristoyltransferase (NMT) with its G protein substrate. Protocols for the production and purification of myristoylated Gα are presented.
TIA742
发表于 2025-3-23 21:39:39
From Tre Styles to Trayvon Martin)-tagged PLC β the purification involves two steps, affinity chromatography with Ni-NTA agarose followed by heparin Sepharose chromatography. 6-His-tagged PLCε can be purified in a single step with nickel nitrilotriacetic acid-agarose (Ni-NTA) affinity chromatography.
TOXIN
发表于 2025-3-24 01:11:38
Incivility as a Tool for Social Change,tion of a phosphatidylalcohol, which is a specific and unique marker for PLD activity. This protocol is highly sensitive for the detection of PLD activity following the stimulation of intact cells, being a valuable method for studying the regulation of PLD activity in vivo.
凝结剂
发表于 2025-3-24 06:02:00
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欺骗世家
发表于 2025-3-24 07:12:48
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Fortify
发表于 2025-3-24 12:04:42
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冰雹
发表于 2025-3-24 15:20:54
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吸气
发表于 2025-3-24 19:51:50
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Headstrong
发表于 2025-3-24 23:15:24
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