HERE
发表于 2025-3-26 23:56:47
https://doi.org/10.1007/978-3-7091-9833-9o driven innovations in protein science. Expressed protein ligation (EPL) is a widely used method to generate chemically modified proteins. However, EPL has some limitations, particularly relevant to modify challenging proteins such as antibodies. The method termed streamlined expressed protein liga
妨碍议事
发表于 2025-3-27 03:47:10
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FEAT
发表于 2025-3-27 05:58:04
Patricia M. Davies MCSP Dip. Phys. Ed.i of proteins. In this way, protein activity can be preserved while functionalizing the target protein with a wide range of chemical handles. Here, we describe methods for EPL-based modification of proteins produced by yeast, employing an engineered, non–self-cleaving intein known as 202-08. Methods
Formidable
发表于 2025-3-27 12:34:07
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Pelvic-Floor
发表于 2025-3-27 15:30:27
https://doi.org/10.1007/978-3-531-92156-3ons form a combinatorial language which defines the functional state of the underlying genome. As each histone type exists in two copies in a nucleosome, the modification patterns can differ between the individual histones, resulting in asymmetry and increasing combinatorial complexity. To systemati
Budget
发表于 2025-3-27 17:50:33
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记忆
发表于 2025-3-28 01:42:46
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变色龙
发表于 2025-3-28 02:49:06
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裤子
发表于 2025-3-28 08:15:07
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scrutiny
发表于 2025-3-28 11:12:29
https://doi.org/10.1007/978-1-0716-0434-2protein engineering; split inteins; C-terminal thioesters; hydrazide oxidation; protein splicing