Insatiable
发表于 2025-3-25 03:27:53
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夹死提手势
发表于 2025-3-25 09:34:13
Identification, Characterization, and Optimization of Split Inteins, the protein splicing reaction, information that can guide further engineering efforts. These techniques provide useful alternatives to common assays that utilize SDS-PAGE to analyze splicing reaction progress.
身心疲惫
发表于 2025-3-25 11:50:15
,Die innere Uhr - Was lässt uns ticken?,N-cysteine containing synthetic peptides in a chemoselective ligation. The recombinant protein C-terminal thioesters are produced by exploiting the action of nature’s inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein li
ENACT
发表于 2025-3-25 15:51:18
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formula
发表于 2025-3-25 23:48:37
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强行引入
发表于 2025-3-26 01:01:57
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septicemia
发表于 2025-3-26 05:30:43
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Rustproof
发表于 2025-3-26 12:18:13
Maarten J. Arentsen,Doris Fuchsession. Peptide hydrazides are widely used as thioester equivalents in the total chemical synthesis and semisynthesis of proteins as they can be easily prepared using standard solid phase peptide synthesis (SPPS) and recombinant peptide techniques. Via treatment with NaNO. and subsequent thiolysis,
清醒
发表于 2025-3-26 16:38:30
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不断的变动
发表于 2025-3-26 19:00:39
https://doi.org/10.1007/978-3-663-20507-4ir ultrafast splicing kinetics allow for the efficient production of native proteins from two separate polypeptides both in vitro and in cells. They can also be used to generate proteins with C-terminal thioesters for downstream applications. In this chapter, we describe a method based on a genetica