有节制
发表于 2025-3-23 11:04:41
Patricia M. Davies MCSP Dip. Phys. Ed. for EPL modification of both yeast surface displayed and secreted proteins with bioorthogonal chemical groups are described. These methods allow for the site-specific modification of intein-fused proteins produced in yeast.
外向者
发表于 2025-3-23 16:13:31
http://reply.papertrans.cn/32/3198/319767/319767_12.png
Ceramic
发表于 2025-3-23 20:15:27
Methods to Study the Structure and Catalytic Activity of ,-Splicing Inteins,ction of inteins can have significant impact for biotechnology applications. Here, we provide biochemical methods to study splicing activity and NMR methods to study intein structure and the catalytic mechanism.
不能妥协
发表于 2025-3-23 22:45:51
Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins,peptide hydrazides can be rapidly converted to peptide thioesters, which then selectively react with recombinant protein containing an N-terminal cysteine (Cys) to form a native peptide bond, thereby linking the two peptide segments without isolating any intermediates.
Proclaim
发表于 2025-3-24 05:29:51
http://reply.papertrans.cn/32/3198/319767/319767_15.png
并置
发表于 2025-3-24 06:45:02
Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation, for EPL modification of both yeast surface displayed and secreted proteins with bioorthogonal chemical groups are described. These methods allow for the site-specific modification of intein-fused proteins produced in yeast.
特征
发表于 2025-3-24 12:02:01
http://reply.papertrans.cn/32/3198/319767/319767_17.png
温和女孩
发表于 2025-3-24 18:43:35
http://reply.papertrans.cn/32/3198/319767/319767_18.png
progestin
发表于 2025-3-24 22:52:55
,Die innere Uhr - Was lässt uns ticken?,tion of nature’s inteins which are protein modules that catalyze protein splicing. This chapter discusses the basic principles of expressed protein ligation and recent advances and applications in this protein semisynthesis field. Comparative strengths and weaknesses of the method and future challenges are highlighted.
无能性
发表于 2025-3-24 23:32:13
https://doi.org/10.1007/978-3-531-90468-9mal ligation sites are discussed. In addition, several methods used to generate the reactive fragments required for EPL are highlighted in practical details. Finally, strategies that one can implement to achieve efficient ligation reactions are presented.