Esophagitis
发表于 2025-3-23 13:44:14
,Der kernbasierte AD-Schätzer KADE,proved to monitor Ca. dynamics in living cells. Here we describe the usage of . resonance energy transfer (FRET)-based Cameleon probes to investigate Ca. influx across the plasma membrane (PM) or Ca. release from the main intracellular Ca. store, the endoplasmic reticulum (ER).
替代品
发表于 2025-3-23 16:42:17
,LEISTUNGSERSTELLUNG IN SÄGEBETRIEBEN,l, using the electrophysiological technique of planar lipid bilayer..We showed that MCU gives rise to single-channel Ca. currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.
弓箭
发表于 2025-3-23 21:10:40
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CUMB
发表于 2025-3-23 23:31:12
Peter Weise,Wolfgang Brandes,Manfred Kraftred contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca. handling in cardiomyocytes represents a central method in experimental molecular cardiology.
Little
发表于 2025-3-24 02:51:08
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品尝你的人
发表于 2025-3-24 08:32:22
Fluchtmigration und berufliche Ausbildung . parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca. assays.
反抗者
发表于 2025-3-24 14:10:36
Neue Migrationen als Herausforderung? One of the most well-established methods to study SOCE is using the Ca.-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-2 to study Ca. signaling from SOCE in human embryonic kidney (HEK) cells.
Wernickes-area
发表于 2025-3-24 18:33:04
,Webern, Schönberg und Strawinsky,ical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.
ADJ
发表于 2025-3-24 22:45:52
,Dieter Schnebel — Heinz Holliger, estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.
贪婪性
发表于 2025-3-25 01:07:15
Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling,proved to monitor Ca. dynamics in living cells. Here we describe the usage of . resonance energy transfer (FRET)-based Cameleon probes to investigate Ca. influx across the plasma membrane (PM) or Ca. release from the main intracellular Ca. store, the endoplasmic reticulum (ER).