牛马之尿
发表于 2025-3-25 05:20:09
MCU Regulation in Lipid Bilayer and Electrophysiological Recording,l, using the electrophysiological technique of planar lipid bilayer..We showed that MCU gives rise to single-channel Ca. currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.
学术讨论会
发表于 2025-3-25 07:40:11
Electrophysiological Characterization of Calcium-Permeable Channels Using Planar Lipid Bilayer,protein complex responsible for the uptake of this ion into mitochondria..Here we describe the protocol used for the electrophysiological characterization of the mitochondrial calcium uniporter (MCU) complex: the following outline indicates step-by-step the setup of planar lipid bilayer experiments.
investigate
发表于 2025-3-25 14:35:35
Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes,red contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca. handling in cardiomyocytes represents a central method in experimental molecular cardiology.
啪心儿跳动
发表于 2025-3-25 16:41:23
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SOBER
发表于 2025-3-25 22:14:22
Employing Transgenic Parasite Strains to Study the Ca, Dynamics in the Human Malaria Parasite ,, . parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca. assays.
fertilizer
发表于 2025-3-26 03:25:22
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投票
发表于 2025-3-26 07:18:36
Assessing Calcium-Stimulated Mitochondrial Bioenergetics Using the Seahorse XF96 Analyzer,ical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.
RALES
发表于 2025-3-26 09:17:46
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盘旋
发表于 2025-3-26 16:02:20
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性满足
发表于 2025-3-26 20:49:11
Textilforschungsanstalt Krefeldle alignment and image acquisition are detailed. Finally, the image processing steps to analyze calcium oscillations are discussed, with particular emphasis on ratiometric calcium imaging in . root hairs.