Benign
发表于 2025-3-30 10:35:09
In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle,n. However, microscopic observation of Ca. signalling in live skeletal muscle tissue has been hampered, in particular, by the combination of the high speed of Ca. transients and the contractile properties that are inherent to muscle. The present chapter describes methods to visualize Ca. signals dur
巧办法
发表于 2025-3-30 14:32:14
TRPML1-/TFEB-Dependent Regulation of Lysosomal Exocytosis,al exocytosis and clearance of lysosomal accumulation in various cellular models of lysosomal storage disorders (LSDs). Here, we described methods to determine TFEB activation and lysosomal exocytosis that may represent innovative tools to study lysosomal function and to develop novel therapeutic ap
nocturia
发表于 2025-3-30 20:00:40
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Scintigraphy
发表于 2025-3-31 00:13:22
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Cabinet
发表于 2025-3-31 02:58:08
Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2,ions. SOCE is mediated through the plasma membrane (PM) protein, Orai1, and the endoplasmic reticulum protein, stromal interaction molecule 1 (STIM1). One of the most well-established methods to study SOCE is using the Ca.-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-
DAUNT
发表于 2025-3-31 07:59:50
Optogenetic Interneuron Stimulation and Calcium Imaging in Astrocytes,st abundant glial cells in the brain. Astrocytes respond to neurotransmitters with Ca. elevations which represent a key event in the modulation of local brain circuits played by these glial cells. Due to technical limitations, the study of Ca. signal dynamics in astrocytes has focused for decades al
男学院
发表于 2025-3-31 09:47:47
Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes,. Reactive oxygen species (ROS) are important factors that influence these redox processes. Calcium ion (Ca.) dynamics and signals are also essential regulators of key cellular processes. Therefore, the combined and precise monitoring of ROS and Ca. in single cells, with a high spatial and temporal
landfill
发表于 2025-3-31 15:02:00
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REIGN
发表于 2025-3-31 19:16:12
Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography ergetic effect of Ca. signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we d
Feigned
发表于 2025-4-1 00:31:57
Anna Raffaello,Denis Vecellio ReaneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts