BLANK
发表于 2025-3-30 09:18:24
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该得
发表于 2025-3-30 13:27:50
Expression and Purification of the CMR (Type III-B) Complex in ,,n or a multi-protein complex. However, expression and purification of one subunit of a complex in the absence of its binding partners has often proven difficult to achieve due to the issues such as instability and mis-folding. This is the case for the components of the CRISPR-Cas interference comple
FIR
发表于 2025-3-30 16:32:46
Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids,lution. The first main step of CRISPR-Cas activity is the immune adaptation through spacer(s) acquisition into an active CRISPR locus. This step is also mandatory for the final stage of CRISPR-Cas activity, namely interference. This chapter describes general procedures for studying the CRISPR adapta
gustation
发表于 2025-3-30 23:18:26
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BRAND
发表于 2025-3-31 02:33:53
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罐里有戒指
发表于 2025-3-31 05:57:24
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sperse
发表于 2025-3-31 09:30:41
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机密
发表于 2025-3-31 16:29:26
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fatty-streak
发表于 2025-3-31 17:43:21
Chemical and Enzymatic Footprint Analyses of R-Loop Formation by Cascade-crRNA Complex,DNA-loop) at the crRNA-complementary sequence (protospacer). After initial unspecific binding to the double-stranded DNA, Cascade-crRNA complex slides along the DNA to find the protospacer. Once the target site is detected, the crRNA hybridizes to the complementary strand with subsequent displacemen
characteristic
发表于 2025-3-31 23:23:07
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