Oscillate
发表于 2025-3-28 15:23:16
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拱形面包
发表于 2025-3-28 20:39:30
In Vitro Co-reconstitution of Cas Protein Complexes,n of the mechanistic details and the protein interaction partners requires production of recombinant Cas proteins. However, these proteins are often produced as inactive inclusion bodies. Here, we present a detailed protocol for the isolation and purification of insoluble Cas proteins. Guidelines fo
bifurcate
发表于 2025-3-29 00:31:32
Analysis of CRISPR Pre-crRNA Cleavage,Cas system by incubation of radiolabeled model RNAs with recombinant CRISPR-associated (Cas) endoribonucleases, followed by denaturing polyacrylamide gel electrophoresis (PAGE) of the products. Determination of cleavage position is based on comparison with RNase T1 digestion and base hydrolysis prod
osteopath
发表于 2025-3-29 05:25:50
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indecipherable
发表于 2025-3-29 10:44:20
Computational Detection of CRISPR/crRNA Targets,ys specifically determines the targets in invader genomes. These spacers provide the short specific RNA nucleotide sequences within the guide crRNAs. In addition to complementarity in the spacer–target (protospacer) interaction, short flanking protospacer adjacent motifs (PAMs), or mismatching flank
生气的边缘
发表于 2025-3-29 11:35:14
High-Throughput CRISPR Typing of , Complex and , Serotype Typhimurium,inical isolates at a phylogeographic level. For other pathogens, such as ., recent studies suggest that specifically designed spoligotyping techniques could be interesting for public health purposes. Spoligotyping was in its original format a reverse line-blot hybridization method using capture prob
tariff
发表于 2025-3-29 16:22:56
Spacer-Based Macroarrays for CRISPR Genotyping, loci in bacteria. To date, it was used almost exclusively for . and was named spoligotyping (spacer oligonucleotides typing). Here, we describe the pipeline of this approach that includes search of loci and selection of spacers, preparation of the membrane with immobilized probes and spoligotyping
细胞
发表于 2025-3-29 21:36:58
Analysis of crRNA Using Liquid Chromatography Electrospray Ionization Mass Spectrometry (LC ESI MS)id chromatography interfaced with electrospray ionization mass spectrometry (LC ESI MS). The direct purification of crRNA from the Cascade-crRNA complex was performed using denaturing ion pair reverse phase chromatography. Following purification of the crRNA, the intact mass was determined by LC ESI
热心
发表于 2025-3-30 00:28:26
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companion
发表于 2025-3-30 08:03:30
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