马笼头
发表于 2025-3-26 22:10:33
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miscreant
发表于 2025-3-27 03:29:36
Adis Alihodzic,Eva Tuba,Milan Tubas for each of which signature genes have been identified. Comparative genomic analysis of the CRISPR-Cas systems in new archaeal and bacterial genomes performed over the 3 years elapsed since the development of this classification makes it clear that new types and subtypes of CRISPR-Cas need to be i
neutrophils
发表于 2025-3-27 06:38:34
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不连贯
发表于 2025-3-27 13:04:24
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Anhydrous
发表于 2025-3-27 15:08:17
Nabil Sabor,Mohammed Abo-Zahhad, suspected as required for this lethal activity. This procedure enabled us to identify a novel gene, ., that is required for the activity of the CRISPR-Cas system. The procedures described here can be adjusted to various organisms to identify genes required for their CRISPR-Cas activity.
heterogeneous
发表于 2025-3-27 21:39:43
Investigating CRISPR RNA Biogenesis and Function Using RNA-seq, a genome-wide annotation of transcriptional start sites. This approach helped to identify a new crRNA biogenesis pathway of Type II CRISPR-Cas systems that involves a trans-encoded small RNA, tracrRNA, and the host factor RNase III.
扩张
发表于 2025-3-27 23:06:02
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indubitable
发表于 2025-3-28 05:15:46
Procedures for Generating CRISPR Mutants with Novel Spacers Acquired from Viruses or Plasmids,as the model system in the first published study on novel spacer acquisition and in many studies ever since, the protocols in this chapter describe specific conditions, media, and buffers that have been used with this microorganism. Details for other species will be given when possible, but readers
清楚
发表于 2025-3-28 09:02:25
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使显得不重要
发表于 2025-3-28 11:01:42
Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function,, suspected as required for this lethal activity. This procedure enabled us to identify a novel gene, ., that is required for the activity of the CRISPR-Cas system. The procedures described here can be adjusted to various organisms to identify genes required for their CRISPR-Cas activity.