nepotism
发表于 2025-3-25 03:53:30
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Abrupt
发表于 2025-3-25 09:58:51
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Hallmark
发表于 2025-3-25 12:41:57
Studies in Computational IntelligenceO.) and the specific cleavage of single-stranded DNA by Nuclease P1 can be used to probe an R-loop formation by Cascade. Localization of the Cascade-crRNA complex on the DNA can be achieved by an Exonuclease III protection assay.
讽刺
发表于 2025-3-25 17:44:30
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搬运工
发表于 2025-3-25 21:11:59
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高射炮
发表于 2025-3-26 01:41:58
https://doi.org/10.1007/978-3-319-13650-9ucts. The mechanism of cleavage is investigated by chemical and enzymatic characterization of the reaction products as well as by the demonstration that a specific 2′-deoxy substitution 5′ to the scissile phosphate blocks endonucleolytic cleavage.
reject
发表于 2025-3-26 06:50:08
Yang Liu,Alan Kwan,Yacine Rezgui,Haijiang Lis have a discriminatory role in accurate target detection. Here, we describe a bioinformatic method, called CRISPRTarget, to use the sequence of a CRISPR array (e.g., predicted via CRISPRDetect/CRISPRDirection) to identify the foreign nucleic acids it targets.
mydriatic
发表于 2025-3-26 09:07:54
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明智的人
发表于 2025-3-26 16:27:47
José M. Araújo,Carlos E. T. Dóreaxes, which degrade invading nucleic acids in a sequence homology-dependent manner in many prokaryotic species. Here, we describe the expression of a tandem-tagged subunit of the Type III-B (CMR) complex in . and subsequent isolation and purification of the whole complex by affinity purification of the tagged subunit.
bifurcate
发表于 2025-3-26 18:39:24
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