多愁善感
发表于 2025-3-21 20:05:11
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清洗
发表于 2025-3-21 22:46:14
Exon Trapping for Positional Cloning and Fingerprinting,ies, or phenotypes, to a quantitative trait locus (QTL), a genomic region no smaller than 1 centiMorgan (cM) or megabase (Mb) in length. Physical mapping can then provide a map of higher resolution. Physical maps are constructed from clones identified by screening genomic libraries. Genomic clones c
初次登台
发表于 2025-3-22 02:47:37
Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column,ize, which are free of methylation and these are known as CpG islands (. . . and . for reviews). In addition to distinctive DNA characteristics, CpG islands have an open chromatin structure in that they are hyperacetylated, lack histone H1, and have a nucleosome-free region (.). The major reason for
文件夹
发表于 2025-3-22 05:46:08
BAC Microarray-Based Comparative Genomic Hybridization,ridization (array CGH) provides a high throughput means to measure and map copy number aberrations on a genome-wide scale and to link them directly to genome sequence. For applications involving the analysis of tumors, the technology must provide reliable detection of single copy gains and losses in
conformity
发表于 2025-3-22 12:31:22
Large DNA Transformation in Plants,). Both methods used modified bacterial artificial chromosomes (BACs) such as BIBAC or pBACwich. BIBAC vector (used in tomato) is capable of transferring large DNA fragments from . into plants. pBACwich (used in tobacco) utilizes a Cre/. site-specific recombination system to integrate large DNA frag
moratorium
发表于 2025-3-22 15:09:56
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Amenable
发表于 2025-3-22 20:28:38
BAC Modification Using a RecA Expressing Shuttle Vector System,BACs have the capacity for maintaining large, often more than 300 kb fragments of mammalian DNA as stable inserts and avoid much of the insert chimerism which characterizes yeast artificial chromosomes (YACs). Isolation of BAC DNA is easy because they exist as supercoiled circular plasmids that are
做作
发表于 2025-3-23 01:17:37
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自爱
发表于 2025-3-23 02:11:19
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馆长
发表于 2025-3-23 08:52:28
BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes,b), which can accommodate most eukaryotic genes along with their full set of regulatory elements, and to their greater convenience in handling over other large cloning vectors like P1-based artificial chromosomes (PACs) and yeast artificial chromosomes (YACs). Two key advances for harnessing the ful