decipher 发表于 2025-3-23 12:03:15
Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes,,.). The first successful attempt to perform this technique was carried out by Lin in 1966 (.), who could show that the early fertilized embryo could survive the mechanical damage of inserting a glass needle into the pronucleus. However, it was not until 1981 that small DNA fragments were integrated多产鱼 发表于 2025-3-23 17:29:07
Generation of BAC Transgenic Mice,acterized “single” genes that contribute to a particular trait. However, single-gene expression in transgenic (.) mice is not appropriate for the functional analysis of the many genes that may reside in a typical genetically mapped locus of the order of 1 cM. Bacterial artificial chromosome (BAC) veGROVE 发表于 2025-3-23 19:56:41
http://reply.papertrans.cn/19/1803/180256/180256_13.png妨碍议事 发表于 2025-3-24 00:11:54
http://reply.papertrans.cn/19/1803/180256/180256_14.pngMonolithic 发表于 2025-3-24 03:40:48
,Cloning of β-Herpesvirus Genomes as Bacterial Artificial Chromosomes,have been completely sequenced, our knowledge on the function of the majority of herpesvirus genes is still quite insufficient. This is especially true for the members of the β-herpesviruses, i.e., the cytomegaloviruses (CMVs), because their slow replication kinetics, cell association, and large genAIL 发表于 2025-3-24 06:30:21
Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors,work of interactions between the virus and the host. Many of these interactions are still unrevealed or not understood in detail. The generation of mutant viruses is an important strategy to obtain insights into the functional role of individual virus genes. A classical genetic approach used chemicaComplement 发表于 2025-3-24 12:05:56
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http://reply.papertrans.cn/19/1803/180256/180256_18.png多余 发表于 2025-3-24 19:26:17
Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies,ruses are subclassified into .-, or .- herpesviruses, dependent upon a variety of criteria. Eight herpesviruses are human pathogens: Herpes Simplex Viruses type 1 and 2; Varicella-Zoster Virus (VZV); Cytomegalovirus (CMV); Human Herpes Viruses type 6 and 7; Epstein-Barr Virus (EBV); and Kaposi’s SarInflated 发表于 2025-3-25 01:42:22
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