书目名称 | Transcriptional Regulation | 副标题 | Methods and Protocol | 编辑 | Ales Vancura | 视频video | | 概述 | Presents detailed protocols developed using mammalian, yeast, and Drosophila cells and tissues, which can easily be applied across species and cell types.Features reliable methods tested in laboratori | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring .in vivo. protein-DNA interactions at any genomic loci. .Transcriptional Regulation: Methods and Protocols. takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world. Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.. .Comprehensive and accessible, .Transcriptional Regulation: Methods and Protocols. equally serves senior researchers and | 出版日期 | Book 2012 | 关键词 | ChIP assays; Chromatin immunoprecipitation; Chromatin structure; Drosophila cells; Gene expression; Histo | 版次 | 1 | doi | https://doi.org/10.1007/978-1-61779-376-9 | isbn_softcover | 978-1-4939-5854-2 | isbn_ebook | 978-1-61779-376-9Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2012 |
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