| 书目名称 | The Genetic Manipulation of Staphylococci | | 副标题 | Methods and Protocol | | 编辑 | Jeffrey L. Bose | | 视频video | | | 概述 | Includes cutting-edge methods and protocols for the study of Staphylococci genetic manipulation.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation ad | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in .Staphylococcus., as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Practical and authoritative, .The Genetic Manipulation of Staphylococci: Methods and Protocols. serves as a vital resource and guide to scientists in the Staphylococcus comm | | 出版日期 | Book 2016 | | 关键词 | Mutant generation; Nucleic acid analysis; Pathogenic bacteria; Protein function; Single nucleotide chang | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-3158-3 | | isbn_softcover | 978-1-4939-4900-7 | | isbn_ebook | 978-1-4939-3158-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2016 |
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发表于 2025-3-21 19:41:41
