书目名称 | TALENs |
副标题 | Methods and Protocol |
编辑 | Ralf Kühn,Wolfgang Wurst,Benedikt Wefers |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | .This volume provides a comprehensive collection of protocols on new technology across various model organisms. Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .TALENs: Methods and Protocols .aims to ensure successful results in the further study of this vital field.. |
出版日期 | Book 2016 |
关键词 | DNA sequence recognition; TAL based gene activators; TAL element DNA binding principles; TALEN coding r |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-2932-0 |
isbn_softcover | 978-1-4939-4905-2 |
isbn_ebook | 978-1-4939-2932-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2016 |