| 书目名称 | Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telom |
| 编辑 | Yusuke Kawamoto |
| 视频video | |
| 概述 | Nominated by Kyoto University as an outstanding Ph.D. thesis.Includes a review chapter summarizing the history of and recent studies on Py–Im.Describes in detail the synthesis of Py–Im polyamides usin |
| 丛书名称 | Springer Theses |
| 图书封面 |  |
| 描述 | .This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole–imidazole (Py–Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py–Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py–Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py–Im polyamides, particularly the extension of the target sites c. |
| 出版日期 | Book 2019 |
| 关键词 | Pyrrole-imidazole Polyamide; Minor Groove Binder; Solid-phase Peptide Synthesis; Telomere; Visualization |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-981-13-6912-4 |
| isbn_ebook | 978-981-13-6912-4Series ISSN 2190-5053 Series E-ISSN 2190-5061 |
| issn_series | 2190-5053 |
| copyright | Springer Nature Singapore Pte Ltd. 2019 |
发表于 2025-3-21 18:24:45
