Titlebook: Stem Cell Assays; Mohan C. Vemuri Book 2007 Humana Press 2007 PCR.RNA.antibody.cancer.cell.electron microscopy.gene expression.gene transf

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Clinical Grade Expansion of Human Bone Marrow Mesenchymal Stem Cells,human body, they are present in various niches, but their main source is bone marrow (BM). The regeneration capability of MSCs, their ease to undergo gene modification, as well as their immunosuppressive capacity render them as popular candidates for tissue engineering, gene therapy, and immunothera

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Feeder-Layer Free Culture System for Human Embryonic Stem Cells, hESCs will require the use of a defined medium and an animal-free culture method that will prevent their possible exposure to animal pathogens. This chapter discusses the advancements in the development of methods for the defined culture of hESCs and describes a simple method for animals serum-free and feeder layer-free culture of hESCs.

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Generation of a Monoclonal Antibody Library Against Human Embryonic Stem Cells,ent method for generating monoclonal antibodies against cell surface antigens expressed by hESCs and stem cells at different stages of differentiation. This method may have profound implications for many aspects of hESC research and therapeutics.

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RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells,small interfering RNA is an effective strategy to knockdown target gene expression, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This method will be useful to test the function of a wide variety of gene products using the ES cell differentiation system.

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Isolation of Stem Cells from Human Umbilical Cord Blood,. Development of reliable methods for isolation and expansion of cord blood stem cells is critical for consequent clinical application. The focus of this chapter is to review the methods currently used by different research groups and to recommend an isolation protocol that yields optimal number of UCB stem cells.