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Titlebook: Stem Cell Assays; Mohan C. Vemuri Book 2007 Humana Press 2007 PCR.RNA.antibody.cancer.cell.electron microscopy.gene expression.gene transf

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发表于 2025-3-21 16:07:47 | 显示全部楼层 |阅读模式
书目名称Stem Cell Assays
编辑Mohan C. Vemuri
视频videohttp://file.papertrans.cn/878/877189/877189.mp4
概述Brings recent developments to the forefront in a timely manner.Presents scientific advances in stem cell methods for a wider use by novice and expert scientists
丛书名称Methods in Molecular Biology
图书封面Titlebook: Stem Cell Assays;  Mohan C. Vemuri Book 2007 Humana Press 2007 PCR.RNA.antibody.cancer.cell.electron microscopy.gene expression.gene transf
描述Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of “transplant-ready” or “clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejec
出版日期Book 2007
关键词PCR; RNA; antibody; cancer; cell; electron microscopy; gene expression; gene transfer; genes; microscopy; mole
版次1
doihttps://doi.org/10.1007/978-1-59745-536-7
isbn_softcover978-1-62703-937-6
isbn_ebook978-1-59745-536-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
The information of publication is updating

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Micro RNA Profiling,s the state of stem cell lines following multiple passages in culture as well as a discovery tool that eliminates the need to screen large numbers of candidate regulatory miRNAs by northern blot or PCR. In this chapter, we describe in detail the method to carry out miRNA array analysis in human embr
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Assays for Alloreactive Responses by PCR,eron-γ and interleukin-2 mRNAs quantification that requires only 10ml of blood. Moreover, results can be obtained within 48h. A modified mixed lymphocyte reaction (MLR) using whole blood T lymphocytes as responsive cells and CD3-depleted peripheral blood mononuclear cells (PBMCs) as stimulators is d
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Book 2007cientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejec
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Carol B. Ware,Szczepan W. Baranance. When the canopy access system was under construction at Lambir Hills - tional Park in the early 1990s, it made use of an underlying technology that was already in place: bridges. For centuries, bridges have spanned the natural chasms over rivers. This existing network of bridges and the people
发表于 2025-3-23 03:24:09 | 显示全部楼层
Micha Drukker,Christina Muscat,Irving L. Weissmanance. When the canopy access system was under construction at Lambir Hills - tional Park in the early 1990s, it made use of an underlying technology that was already in place: bridges. For centuries, bridges have spanned the natural chasms over rivers. This existing network of bridges and the people
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