Titlebook: Staphylococcus Epidermidis; Methods and Protocol Paul D. Fey Book 2014 Springer Science+Business Media, LLC 2014 Staphylococcus Epidermidis

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Methods to Generate a Sequence-Defined Transposon Mutant Library in , Strain 1457,late. Although sequence-defined transposon mutant libraries have been constructed in ., no such library exists for .. .. Nevertheless, the study of Tn.-mediated mutations has been paramount in discovering unique aspects of . biology including initial adherence and accumulation during biofilm formati

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Examination of , Biofilms Using Flow-Cell Technology, flow of nutrients. Under these conditions, the bacteria progress through a series of developmental steps, ultimately forming a multicellular structure containing differentiated cell populations. The observation of the biofilm at various time-points throughout this process provides a glimpse of the

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Rapid Quantitative and Qualitative Analysis of Biofilm Production by , Under Static Growth Conditioal. in 1985 is fast and does not require specialized instruments. Thus, laboratories with standard microbiology infrastructure and a 96-well plate reader can easily use this technique to generate data on the biofilm phenotypes of multiple . strains and clinical isolates. Furthermore, this method can

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Bacteriophage Transduction in ,,ain construction are laborious and hampered by poor efficiency. The ability to move chromosomal genetic markers and plasmids using bacteriophage transduction has greatly increased the speed and ease of . studies. These molecular genetic advances have advanced the . research field beyond a select few

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A Mouse Model of , Catheter-Associated Biofilm Infection,ciated biofilm infections in humans that are highly recalcitrant to antimicrobial therapy and alter host immune responses to facilitate bacterial persistence. Here we describe a mouse model of catheter-associated biofilm infection, which can be utilized to investigate the importance of various staph

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