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Titlebook: Sphingosine-1-Phosphate; Methods and Protocol Alice Pébay,Kursad Turksen Book 2018Latest edition Springer Science+Business Media, LLC 2018

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书目名称Sphingosine-1-Phosphate
副标题Methods and Protocol
编辑Alice Pébay,Kursad Turksen
视频video
概述Includes cutting-edge techniques for the study of sphingosine-1-phosphate.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts.Includes s
丛书名称Methods in Molecular Biology
图书封面Titlebook: Sphingosine-1-Phosphate; Methods and Protocol Alice Pébay,Kursad Turksen Book 2018Latest edition Springer Science+Business Media, LLC 2018
描述.This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in developmentand in physiopathology.  As a volume in the .Methods in Molecular Biology. series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls...Authoritative and timely, .Sphingosine-1-Phosphate: Methods and Protocols, Second Edition. is another key resource for scientists working in this fast-moving and dynamic field..
出版日期Book 2018Latest edition
关键词Immunohistochemical; Physiopathology; Sphingolipids; S1P; Bioactive lysophospholipid; Stem cells
版次2
doihttps://doi.org/10.1007/978-1-4939-7413-9
isbn_softcover978-1-4939-8478-7
isbn_ebook978-1-4939-7413-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2018
The information of publication is updating

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Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Eed in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.
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Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation,te-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.
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Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Farowth factor (PDGF). This complete protocol provides a simple alternative chemically defined serum-free system that is relatively inexpensive and advantageous for studying signaling pathways involved in hESC pluripotency.
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