Titlebook: Single-Domain Antibodies; Methods and Protocol Greg Hussack,Kevin A. Henry Book 2022 The Editor(s) (if applicable) and The Author(s), under

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Tosha M. Laughlin,James R. Hornnierenden Lösungsweg wegführt. Jedenfalls werden aber die entscheidenden Punkte in der Lösung hervorgehoben und detailliert beschrieben. Nach Möglichkeit sind alle Argumentationsschritte aufgeführt, sodass Sie dem Lösungsstrang in aller Regel gut folgen können sollten.

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Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targetsct with the magnetized target cells can be isolated using a magnet. Here, we detail protocols for the isolation of binders to membrane protein targets from a yeast display nanobody library using magnetized yeast cell targets. We provide guidance on how to generate magnetic yeast cell targets as well

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A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Acribe a protocol for obtaining monoclonal HCAbs from immunized Harbour transgenic mice through the generation and screening of HEK cell libraries of secreted antibodies. The method can be used routinely and is fast and affordable for everyone. Selected VH regions (single domains) are sequenced and i

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1064-3745 oting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .Single-Domain Antibodies: Methods and Protocols .aims to be a useful, practical guide to help researchers further their studies in this field..978-1-0716-2077-9978-1-0716-2075-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Frédéric Trempe,Martin A. Rossotti,Tahir Maqbool,C. Roger MacKenzie,Mehdi Arbabi-Ghahroudi

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Jahaziel Gasperin-Bulbarela,Olivia Cabanillas-Bernal,Salvador Dueñas,Alexei F. Licea-Navarro