Titlebook: Single-Cell Assays; Microfluidics, Genom Paul C.H. Li,Angela Ruohao Wu Book 2023 The Editor(s) (if applicable) and The Author(s), under exc

Intrepid

crucial

Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis,eneity within cell populations. Here the design and use of a dielectrophoresis-assisted self-digitization (SD) microfluidics chip is described; it captures single cells in isolated microchambers with high efficiency for single-cell analysis. The self-digitization chip spontaneously partitions aqueou

Seminar

Optical pH Monitoring in Microdroplet Platforms for Live Cell Experiments Using Colloidal Surfactanity for emulsified monodisperse droplets to carry out compartmentalization. Fluorinated silica-based nanoparticles are used; they can minimize crosstalk in microdroplets and provide further functionalities by surface labeling. Here we describe a protocol for monitoring pH changes in live single cell

Gastric

PicoShells: Hollow Hydrogel Microparticles for High-Throughput Screening of Clonal Libraries,ction chambers which can be utilized for various chemical assays or reactions. Here we describe the use of a microfluidic droplet generator to encapsulate single cells within hollow hydrogel microparticles called PicoShells. The PicoShell fabrication utilizes a mild pH-based crosslinking modality of

Intersect

Single-Cell Micropatterning by Non-fouling Hydrogels,ll-adherent peptide surrounding by non-fouling cell-repellent poly(ethylene glycol) (PEG) hydrogel, this patterning method allows for controlling cell attachment with desired sizes and shapes up to 19 days. Here we provide the detailed procedure of fabrication for such patterns. This method will all

Hippocampus

Microfluidics-Enabled Isolation and Single-Cell Analysis of Circulating Tumor Cells,d theragnosis. Combined with immunocytochemistry/immunofluorescence (ICC/IF) assays for CTCs, microfluidics-enabled detection presents a unique opportunity to study tumor heterogeneity and predict treatment response, both of which can help cancer drug development. In this chapter, we detail the prot

Foam-Cells

Discrimination of Multidrug Resistance in Cancer Cells Achieved Using Single-Cell Analysis,e is an ever-growing need for advanced methods to study and analyze the response of cancer cells to therapeutic intervention. To determine the cancer cells and responses to various cancer therapies, from a cell mortality perspective, we report a label-free and real-time method to monitor the in situ

organism

厚颜无耻

A High-Throughput Single-Cell Assay on a Valve-Based Microfluidic Platform Applied to Protein Quantnitoring, and drug discovery. Because of the details of information that can be obtained at single-cell resolution, the single-cell assay has been applied to tackle challenging issues such as cancer treatment. Information like the levels of protein expression, cellular heterogeneity, and unique beha

Notify