Titlebook: Single Molecule Analysis; Methods and Protocol Erwin J. G. Peterman Book 2018Latest edition Springer Science+Business Media, LLC, part of S

爆米花

engrave

Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopyeletal structures, such as microtubules and actin, with great detail. For example, Single-Molecule Localization Microscopy (SMLM) achieves resolutions of 5–50 nm through repetitive sparse labeling of samples, followed by Point-Spread-Function analysis of individual fluorophores. Whereas initially th

MAPLE

Parabola

Control-Group

Atomic Force Microscopy: An Introductionount to investigate structures at the single particle level. Often two-dimensional images are not sufficient and further information such as topography and mechanical properties are required. Furthermore, to increase the biological relevance, it is desired to perform the imaging in close to physiolo

专心

Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopyicate and repair DNA. Scanning (or atomic) force microscopy is an ideal tool for this, providing 3D information on molecular structure at nm resolution from defined components. This is a convenient and practical addition to in vitro studies as readily obtainable amounts of purified proteins and DNA

窝转脊椎动物

护身符

歹徒

foppish

Single-Molecule Fluorescence Microscopy in Living ,end, we employ standard fluorescent proteins, an epi-illuminated, wide-field fluorescence microscope and mostly open-source software. This chapter describes the setup we use, the preparation of samples, a protocol for single-molecule imaging in . and data analysis.