Titlebook: Shotgun Proteomics; Methods and Protocol Daniel Martins-de-Souza Book 2014 Springer Science+Business Media New York 2014 High throughput id

旧石器时代

Shotgun Proteomics978-1-4939-0685-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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下级

https://doi.org/10.1007/978-1-4939-0685-7High throughput identification; Label-free proteomics; Mass spectrometry; Post-translational modificati

Conflagration

IPG Strip-Based Peptide Fractionation for Shotgun ProteomicsThe separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible.

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Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL) (ICPL) as part of a shotgun proteomics workflow denoting its advantages and disadvantages. This protocol is suitable to studying any proteome of interest, only requiring a specific sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders.

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Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscled even a lesser number quantified, each with its own weaknesses and strengths. Presented here are the “tandem mass tags” isobaric labels (TMT) and a protocol for its utilization in the quantification of skeletal muscle, which might be adapted also to other animal tissues.

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