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Titlebook: Sexually Transmitted Diseases; Rosanna W. Peeling,P. Frederick Sparling Book 1999 Humana Press 1999 infectious diseases

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Genotyping , by PCRrget for serotyping is the major outer membrane protein (MOMP) which is the most antigenically diverse and abundant surface protein of the organism. Polyclonal antibodies (PAbs) were initially used for typing and were able to identify serovars D through K and LI, L2, and L3 as primarily genital path
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Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS)mprovement in our understanding of the natural history of HIV-1/AIDS. A number of recent studies have convincingly demonstrated that high levels of viral replication occur at all stages of disease (.–.), and that changes in viral RNA load are predictive of disease outcome (.,.) and response to thera
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Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCRn the form of cell-free or cell-associated HIV-1 RNA in semen are important for epidemiologic and public health aspects of the epidemic. Earlier studies used viral culture to detect HIV in semen. Cell associated culturable virus recovery rates ranged from 8 to 55% (.–.). Much lower recovery rates (3
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Detection of Genital Mycoplasmas by PCRr orders, five families, and eight genera, is shown in . (.). The term ‘mollicute’ is sometimes used trivially to describe any organism in the class. The term ‘mycoplasma’ might be used best to describe any member of the genus ., but is also used, as in this chapter, in a trivial way to refer to any organism in the class.
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Hepatitis B Virusons which include: cirrhosis, hepatic failure, hepatocellular carcinoma, as well as extrahepatic manifestations, such as vasculitis and membranoproliferative glomerulonephritis. It is estimated that there are currently 350 million chronic HBV carriers worldwide (.).
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Molecular Diagnosis of HIV-1 by PCRA and Western blot are now extremely reliable, with sensitivities of 99.5% and specificities of 99.8% for the diagnosis of HIV-1 (.). These tests are fairly rapid, reproducible across laboratories, well quality-controlled, relatively inexpensive, and form the basis of the protection of the blood supply.
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