Titlebook: SARS-CoV-2; Methods and Protocol Justin Jang Hann Chu,Bintou Ahmadou Ahidjo,Chee Ke Book 2022 The Editor(s) (if applicable) and The Author(

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Bioinformatics-Based Approaches to Study Virus–Host Interactions During SARS-CoV-2 Infectiongy. Recent technologies in high-throughput sequencing are providing the swift generation of SARS-CoV-2 genomic data with the basic inside of viral infection. Owing to various virus–host protein interactions, high-throughput technologies are unable to provide complete details of viral pathogenesis. I

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Human Nasal Epithelial Cells (hNECs ) Generated by Air-Liquid Interface (ALI) Culture as a Model SysRS-CoV-2 virus responsible for the ongoing COVID-19 pandemic. Here, we describe in detail methods for in vitro ALI differentiation of primary cells collected from human donors, to obtain differentiated hNECs. This can serve as a physiologically relevant model to investigate various aspects of host–p

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Animal Models of COVID-19: Nonhuman Primatesd was going chaotic, the researchers throughout the world were struggling to contribute to the best of their capabilities to advance the understanding of this new pandemic and fast track the development of novel therapeutics and vaccines. While various animal models have helped a lot to understand t

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Animal Models of COVID-19: Transgenic Mouse ModelWuhan, China, and rapidly spread throughout the world, threatening global public health. An animal model is a valuable and a crucial tool that allows understanding of nature in the pathogenesis of SARS-CoV-2 and its associated COVID-19 disease. Here we introduce detailed protocols of SARS-CoV-2 infe

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Propagation and Quantification of SARS-CoV-2ne and the respiratory epithelial Calu-3 cell line. Additionally, we provide protocols for the quantification of SARS-CoV-2 by plaque assays and immunofocus forming assays in Vero E6 cells utilizing liquid overlays. These protocols provide a foundation for laboratories acquiring the ability to study SARS-CoV-2 to address this ongoing pandemic.

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