Titlebook: Retinal Development; Methods and Protocol Shu-Zhen Wang Book 2012 Springer Science+Business Media, LLC 2012 DNA methylation.ERG (function)

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Generation of Transgenic , Using Restriction Enzyme-Mediated Integrationiseases and to study the function and/or regulation of genes. Generating transgenic . is rapid and involves simple in vitro manipulations, taking advantage of the large size of the amphibian egg and external embryonic development. Restriction enzyme-mediated integration (REMI) has a number of advant

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In Vivo Functional Analysis of Transcription Factor: Response Element Interaction Using Transgenic ,ent involves analysis of the functional interaction between a .-acting factor and its corresponding .-acting element in the context of a target promoter in vivo. We describe a method for this analysis in transgenic . tadpoles in which expression of the .-acting factor is knocked down using an shRNA-

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Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in , Embryosfate map, one can specifically modulate levels of gene expression in retina lineages to determine the function of proteins in various aspects of early retinal development, such as formation of the eye fields and determination of specific cell fates. This chapter presents the techniques for identifyi

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Testing Retina Fate Commitment in , by Blastomere Deletion, Transplantation, and Explant Culture precursors of the retina. Using this retina fate map, one can test the commitment of each of these cells to various retinal cell fates by manipulating the environment in which they develop. This chapter presents the techniques for identifying specific retina blastomere precursor cells, deleting the

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Application of Cre-loxP Recombination for Lineage Tracing of Adult Zebrafish Retinal Stem Cellsused in mice, this technology has only recently been applied to zebrafish. Here we describe Cre-loxP methodology for conditional expression of transgenes in zebrafish and their use in lineage tracing Müller glia as they undergo cellular reprogramming and proliferation to repair damaged retinal circu