Titlebook: Retinal Degeneration; Methods and Protocol Bernhard H. F. Weber,Thomas Langmann Book 2019Latest edition Springer Science+Business Media, LL

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1064-3745 eshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Retinal Degeneration: Methods and Protocols, Second Edition .aims to ensure successful scientific work in the further study of this vital field..978-1-4939-9360-4978-1-4939-8669-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Elso describe some useful applications of the protocol, e.g., generation of rod- or cone-enriched retinal organoids, AAV transfection, and cell birth dating. In addition, we provide details of how to process retinal organoids for single organoid gene expression analysis, immunohistochemistry, and electron microscopy.

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Book 2019Latest editionological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment a

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Conduct and Quality Control of Differential Gene Expression Analysis Using High-Throughput Transcripd-sclera) at two different locations (periphery and macular region) from eight individuals. The procedure described in this chapter will use various programs from the free, open-source . software package to analyze sequencing data and to ascertain genes that are differentially expressed between retina and RPE-choroid-sclera.

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Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cellsnctional cells that display many features of native RPE. Here, I describe a protocol for the generation of iPSC-derived RPE monolayer, their propagation, and cryostorage. A reliable monitoring for functional cell differentiation is achieved by measuring transepithelial resistance.