Titlebook: Regulatory Non-Coding RNAs; Methods and Protocol Gordon G. Carmichael Book 2015 Springer Science+Business Media New York 2015 Fluorescence

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FANG

Locating RNAs In Situ with FISH-STIC Probes,e a straightforward means for laboratories to design their own FISH probes that can be synthesized commercially. Here we provide a detailed protocol for applying FISH-STIC probes for in situ hybridization on cultured cells as a convenient and flexible method for localizing individual RNAs with many fluorophores using fluorescence microscopy.

Density

Accrue

Sequence-Defined Oligoaminoamides for the Delivery of siRNAs, displayed efficient siRNA-mediated gene silencing in vitro and in vivo. In this chapter, we provide a brief background on the special features of these polymers and detailed protocols to evaluate polyplex formation, gene silencing efficiency, and cytotoxicity of siRNA-containing polyplexes.

不满分子

forecast

Identification and Validation of miRNA Target Sites Within Nontraditional miRNA Targets, predicting target sites within these RNAs. Here, the principles for developing computational algorithms for identifying putative miRNA target sites outside of mRNA are discussed. Laboratory techniques for validating computational miRNA target predictions are described.

Negligible

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nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale,nteraction between RNA and chromatin modifiers, Polycomb proteins, as an example to describe this method. Using nRIP-seq, we provide a snapshot of Ezh2, a Polycomb component, and RNA interaction in mouse embryonic stem cells.

Graduated

Diverse Functions and Mechanisms of Mammalian Long Noncoding RNAs,iological processes. Here, we discuss their discovery, features, and known functions in cells. While not comprehensive, this chapter should serve to illustrate the power and promise of studying long noncoding RNAs.

heartburn

Sequence-Defined Oligoaminoamides for the Delivery of siRNAs,s attention as a tool for gene silencing in basic science and as a novel therapeutic modality. To develop carriers for cytosolic and systemic siRNA delivery, our laboratory has recently developed a sequence-defined polymer platform compatible with solid-phase-supported synthesis. These polymers have