Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2024Latest edition The Editor(s) (if applicabl

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High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains,tion, and instability. Cell-surface receptor ectodomains are more amenable to large-scale production, but this requires designing and testing various truncation constructs. However, since each protein is unique, testing these constructs individually for many targets is a time-consuming process. In t

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Generation of Monoclonal Chinese Hamster Ovary Cell Lines Using DISPENCELL-S3,terization of an adequate cell sample for single-cell isolation, as well as the optimization of the DISPENCELL-S3 dispensing parameters are described. Monoclonal outgrowth assessment and the use of the recorded impedance signal as evidence of clonality are also outlined.

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The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Productitep-by-step description of the protocols we use for the generation of the recombinant BacMam viruses, the transduction of mammalian cell cultures, and optimization of the protein production conditions through small-scale expression and purification tests.

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,A Versatile Method for Inducible Protein Production in 293 Cells Using the PiggyBac Transposon Systmethodology is based on the . transposon system and enables the inducible production of the protein of interest. Finally, this methodology can easily be used in conventional laboratory cell culture settings without requiring specialized devices.

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Expression of Multispecific Antibodies,es using the BEAT. interface. This interface allows to generate antibodies with very high levels of heterodimer product (reported titers exceed 10 g/L) and comes with a built-in purification strategy allowing removal of residual levels of undesired product-related impurities (e.g., homodimers and half molecules).